Conducting Line transect-based one-dimensional nearest neighbor distance and conducting hypothesis testing related to local distributional aggregation pattern of species. The reason that such a package is needed is because traditional two-dimensional nearest neighbor distance is not applicable when biodiversity data are sampled via optimal ecological survey methods, like line transects. In comparison to the entire studied region, line transect-collected local biodiversity data are spatially constrained and sampling-limited. To this end, two-dimensional nearest neighbor distance would tend to over-estimate distributional aggregation pattern of species when using this limited biodiversity information. Accordingly, one-dimensional nearest neighbor distance is needed and the associated statistical testing should be established for analyzing line transect-derived biodiversity data.