Dithionite Scramblase Assay Analysis

The lipid scrambling activity of protein extracts and purified scramblases is often determined using a fluorescence-based assay involving many manual steps. flippant offers an integrated solution for the analysis and publication-grade graphical presentation of dithionite scramblase assays, as well as a platform for review, dissemination and extension of the strategies it employs. The package's name derives from a play on the fact that lipid scrambling is also sometimes referred to as 'flipping'.

An R package for the automated analysis of dithionite scramblase assays.

To install the development version, you first need the devtools package.


Then you can install the flippant package using


A stable version is planned to be made available via Bioconductor.

The manuscript

The manuscript is provided in Rmd form in the inst/manuscript directory. Note that building the manuscript takes around an hour, since it runs the whole analysis.

The dataset

The original data in contained in a zip file in the inst/extdata directory. Each file within the zip archive is a text file. Lines 4 through to the penultimate line contain tab delimited text data.


scramblase_assay_traces draws time series of relative fluorescence vs. acquisition time, possibly split by experiment.

scramblase_assay_plot draws plots of the probability of at least one flippase vs. the protein to phospholipid ratio.

scramblase_assay_stats calculates the fit constant for each experiment.

scramblase_assay_calculations provides a lower-level overview of model fit details.


Reference manual

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1.1.0 by Johannes Graumann, 2 months ago

Browse source code at https://github.com/cran/flippant

Authors: Johannes Graumann [cre, aut], Richard Cotton [ctb]

Documentation:   PDF Manual  

GPL (>= 3) license

Imports assertive.files, assertive.numbers, assertive.properties, assertive.strings, assertive.types, data.table, magrittr, minpack.lm, plyr, RcppRoll, stringi, utils, withr, wmtsa

Depends on ggplot2

Suggests bookdown, devtools, microbenchmark, multipanelfigure, pander, roxygen2, tools

See at CRAN